首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素-36γ(IL1F9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL1F9含量。IL1F9是重要靶点。它属于IL-1家族，在免疫调节等生理过程中扮演角色。研究机制方面，围绕其与相关信号通路的相互作用展开，探索其在疾病发生发展中的功能，有望为免疫相关疾病的治疗提供新方向。试剂盒检测范围为78 pg/mL-5000 pg/mL，本试剂盒可广泛应用于体外实验中探究IL - 36γ在免疫调节、炎症机制中的功能，支持科研人员分析不同处理条件下（如药物干预或基因修饰）细胞因子分泌动态，或评估疾病模型中IL - 36γ的表达变化。其兼容多种生物样本的特性，为体外研究提供了从基础细胞实验到组织水平检测的完整解决方案，特别适用于炎症相关信号通路研究、生物标志物筛选及药物作用机制探索等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL 1 epsilon ELISA Kit; IL 1 related protein 2 ELISA Kit; IL 1(EPSILON) ELISA Kit; IL 1F9 ELISA Kit; IL 1H1 ELISA Kit; IL 1RP2 ELISA Kit; IL-1 epsilon ELISA Kit; IL-1-related protein 2 ELISA Kit; IL-1F9 ELISA Kit; IL-1H1 ELISA Kit; IL-1RP2 ELISA Kit; IL1E ELISA Kit; Il1f9 ELISA Kit; IL1F9_HUMAN ELISA Kit; IL1H1 ELISA Kit; IL1RP2 ELISA Kit; IL36 gamma ELISA Kit; IL36G ELISA Kit; Interleukin 1 epsilon ELISA Kit; Interleukin 1 family member 9 ELISA Kit; Interleukin 1 homolog 1 ELISA Kit; Interleukin 1 related protein 2 ELISA Kit; Interleukin 36 gamma ELISA Kit; Interleukin-1 epsilon ELISA Kit; Interleukin-1 family member 9 ELISA Kit; Interleukin-1 homolog 1 ELISA Kit
缩写：IL1F9
Uniprot No.：Q9NZH8
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-99 | |||||
回收率：
| The recovery of human IL1F9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 86-98 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.0521.9522.0021.89825001.6111.4971.5541.45012501.2421.2041.2231.1196250.8140.7570.7860.6823120.5830.5380.5610.4571560.3130.2890.3010.197780.2010.1990.2000.09600.1070.1010.104 | ||||||
货期：3-5 working days
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