首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素37(IL-37)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的IL37含量。IL37是一种细胞因子，属于IL-1家族，具有抑制炎症反应等作用。其研究机制主要围绕在调节免疫细胞功能、拮抗促炎信号通路等方面，通过调控多种信号转导途径来影响机体免疫稳态，在炎症性疾病等领域研究较多。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本试剂盒适用于基础科研领域，如炎症机制探索、疾病模型构建、药物干预效果评估等研究方向，尤其支持体外细胞实验（如免疫细胞刺激模型）或动物模型样本中IL-37的动态监测，为免疫调控相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FIL1 ELISA Kit; FIL1 zeta ELISA Kit; FIL1(ZETA) ELISA Kit; FIL1Z ELISA Kit; IL 1 zeta ELISA Kit; IL 1F7 ELISA Kit; IL 1F7b (IL 1H4; IL 1H; IL 1RP1) ELISA Kit; IL 1H4 ELISA Kit; IL 1RP1 ELISA Kit; IL 1X ELISA Kit; IL 1X protein ELISA Kit; IL-1 zeta ELISA Kit; IL-1F7 ELISA Kit; IL-1H ELISA Kit; IL-1H4 ELISA Kit; IL-1RP1 ELISA Kit; IL-1X ELISA Kit; IL-37 ELISA Kit; IL1F7 (canonical product IL 1F7b) ELISA Kit; IL1F7 ELISA Kit; IL1F7_HUMAN ELISA Kit; IL1H4 ELISA Kit; IL1RP1 ELISA Kit; Interleukin 1 family member 7 ELISA Kit; interleukin 1 family; member 7 (zeta) ELISA Kit; Interleukin 1 homolog 4 ELISA Kit; Interleukin 1 related protein ELISA Kit; Interleukin 1 superfamily z ELISA Kit; Interleukin 1 zeta ELISA Kit; Interleukin 37 ELISA Kit; Interleukin-1 family member 7 ELISA Kit; Interleukin-1 homolog 4 ELISA Kit; Interleukin-1 zeta ELISA Kit; Interleukin-1-related protein ELISA Kit; Interleukin-23 ELISA Kit
缩写：IL1F7
Uniprot No.：Q9NZH6
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-37 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 87 | |||||||||||
| Range % | 81-93 | ||||||||||||
| 1:2 | Average % | 85 | |||||||||||
| Range % | 80-92 | ||||||||||||
| 1:4 | Average % | 95 | |||||||||||
| Range % | 90-100 | ||||||||||||
| 1:8 | Average % | 98 | |||||||||||
| Range % | 88-103 | ||||||||||||
回收率：
| The recovery of human IL-37 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 92 | 86-100 | |||||||||||
| EDTA plasma (n=4) | 94 | 84-98 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| pg/mlOD1OD2AverageCorrected20001.6541.6051.6301.46810001.2191.2331.2261.0645000.8540.8920.8730.7112500.5620.5810.5720.4101250.3590.3790.3690.20762.50.2680.2800.2740.11231.250.2180.2130.2160.05400.1650.1590.162 | |||||||||||||
货期：3-5 working days
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