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Human Interleukin-36 receptor antagonist protein(IL1F5) ELISA kit

  • 中文名: 人白介素-36受体拮抗蛋白(IL1F5)酶联免疫试剂盒
货号: IPD-EL011616HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人白介素-36受体拮抗蛋白(IL1F5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的IL1F5含量。IL1F5属于白细胞介素 -1家族成员。其在炎症调节等生理病理过程中有潜在作用。研究机制主要围绕其信号通路展开,它可能通过与特定受体结合,激活下游信号级联反应,影响细胞的增殖、分化及炎症因子的释放等,但仍有许多细节待深入探究。试剂盒检测范围为31.25 pg/ml- 2000 pg/ml,提供预包被抗体及标准品,操作流程标准化,适用于体外研究IL - 36信号通路调控机制、评估炎症性疾病模型中的蛋白表达变化,或筛选靶向IL - 36通路的候选药物分子。科研人员可通过该产品深入探究IL - 36Ra在皮肤屏障功能、先天免疫应答及慢性炎症疾病中的生物学作用,为相关领域的基础研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    AI413231 ELISA Kit; Family of interleukin 1 delta ELISA Kit; FIL1 ELISA Kit; FIL1 delta ELISA Kit; FIL1(DELTA) ELISA Kit; FIL1D ELISA Kit; Fil1delta ELISA Kit; I36RA_HUMAN ELISA Kit; IL 1 delta ELISA Kit; IL 1 related protein 3 ELISA Kit; IL 1F5 (IL 1HY1, FIL1 delta, IL 1RP3, IL 1L1, IL 1 delta) ELISA Kit; Il 1h3 ELISA Kit; IL 1HY1 ELISA Kit; IL 1L1 ELISA Kit; IL 1ra homolog ELISA Kit; IL 1ra homolog, IL1F5 (Canonical product IL 1F5a) ELISA Kit; IL 1RP3 ELISA Kit; IL-1 delta ELISA Kit; IL-1-related protein 3 ELISA Kit; IL-1F5 ELISA Kit; IL-1HY1 ELISA Kit; IL-1L1 ELISA Kit; IL-1ra homolog 1 ELISA Kit; IL-1RP3 ELISA Kit; IL1F5 ELISA Kit; IL1HY1 ELISA Kit; IL1L1 ELISA Kit; IL1RP3 ELISA Kit; IL36RA ELISA Kit; IL36RN ELISA Kit; Interleukin 1 delta ELISA Kit; Interleukin 1 family member 5 (delta) ELISA Kit; Interleukin 1 family member 5 ELISA Kit; Interleukin 1 HY1 ELISA Kit; Interleukin 1 like protein 1 ELISA Kit; Interleukin 1 receptor antagonist homolog 1 ELISA Kit; Interleukin 36 receptor antagonist ELISA Kit; Interleukin-1 delta ELISA Kit; Interleukin-1 family member 5 ELISA Kit; Interleukin-1 HY1 ELISA Kit; Interleukin-1 receptor antagonist homolog 1 ELISA Kit; Interleukin-1-like protein 1 ELISA Kit; Interleukin-36 receptor antagonist protein ELISA Kit; MGC29840 ELISA Kit; PSORP ELISA Kit; RP23-176J12.6 ELISA Kit
  • 缩写:
    IL1F5
  • Uniprot No.:
    Q9UBH0
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    31.25 pg/ml - 2000 pg/ml
  • 灵敏度:
    7.8 pg/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    85


    Range %

    81-93


    1:2

    Average %

    90


    Range %

    85-96


    1:4

    Average %

    95


    Range %

    90-100


    1:8

    Average %

    93


    Range %

    88-97


  • 回收率:

    The recovery of human IL1F5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-99


    EDTA plasma (n=4)

    94

    89-98


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.175

    2.082

    2.129

    2.025


    1000

    1.733

    1.597

    1.665

    1.561


    500

    1.289

    1.209

    1.249

    1.145


    250

    0.838

    0.784

    0.811

    0.707


    125

    0.536

    0.501

    0.519

    0.415


    62.5

    0.371

    0.354

    0.363

    0.259


    31.25

    0.196

    0.191

    0.194

    0.090


    0

    0.106

    0.102

    0.104




  • 货期:
    3-5 working days

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