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产品描述：人白介素36α(IL1F6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的IL36A含量。IL36A属于IL-1细胞因子家族成员，在炎症反应等生理病理过程中扮演重要角色。研究发现它可通过与特定受体结合，激活下游信号通路，参与调控免疫细胞的活性与功能，在炎症性疾病等研究中是潜在作用靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于体外实验研究，可广泛应用于炎症性疾病机制探索、药物靶点筛选、免疫调节研究等领域，例如银屑病动物模型中的细胞因子动态监测、体外药物干预后炎性因子表达水平评估等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FIL1 ELISA Kit; FIL1 epsilon ELISA Kit; FIL1(EPSILON) ELISA Kit; FIL1E ELISA Kit; IL 1 epsilon ELISA Kit; IL 1F6 ELISA Kit; IL 1H1 ELISA Kit; IL-1 epsilon ELISA Kit; IL-1F6 ELISA Kit; IL1E ELISA Kit; IL1F6 ELISA Kit; IL1F6_HUMAN ELISA Kit; IL1RP2 ELISA Kit; IL36 alpha ELISA Kit; IL36A ELISA Kit; Interleukin 1 epsilon ELISA Kit; Interleukin 1 family member 6 (epsilon) ELISA Kit; Interleukin 1 family member 6 ELISA Kit; Interleukin 36 alpha ELISA Kit; Interleukin-1 epsilon ELISA Kit; Interleukin-1 family member 6 ELISA Kit; MGC129552 ELISA Kit; MGC129553 ELISA Kit; MGC151479 ELISA Kit; MGC151481 ELISA Kit; OTTMUSP00000012798 ELISA Kit; RP23-176J12.4 ELISA Kit
缩写：IL1F6
Uniprot No.：Q9UHA7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 80-95 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human IL1F6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 86-98 | ||||
| EDTA plasma (n=4) | 97 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10001.9661.9571.9621.8615001.4321.5011.4671.3662500.9500.9200.9350.8341250.6800.6890.6850.58462.50.4650.5020.4840.38331.20.2750.2870.2810.18015.60.1820.1780.1800.07900.0980.1030.101 | ||||||
货期：3-5 working days
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