首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素36β(IL1F8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL1F8含量。IL1F8属于IL-1家族成员。其背景在于在免疫调节等生理过程有潜在作用。研究机制方面，主要聚焦于它在细胞信号传导路径中的角色，可能通过与相应受体结合调控免疫细胞功能，影响炎症反应等，是免疫学研究的潜在靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外检测不同来源样本中IL - 36β的表达水平，为研究炎症性疾病机制、药物筛选或免疫调控实验提供可靠工具；广泛应用于基础科研领域，例如银屑病动物模型研究、炎症因子网络分析或免疫治疗相关细胞实验，为探索IL - 36β在疾病微环境中的功能及潜在干预靶点提供技术支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2310043N20Rik ELISA Kit; Family of interleukin 1 eta ELISA Kit; FIL1 (ETA) ELISA Kit; FIL1 ELISA Kit; FIL1 eta ELISA Kit; FIL1H ELISA Kit; FILI-(ETA) ELISA Kit; IL 1F8 (FIL1 eta ELISA Kit; IL 1F8 ELISA Kit; IL 1H2 ELISA Kit; IL-1 eta ELISA Kit; IL-1F8 ELISA Kit; IL-1H2 ELISA Kit; IL1 ETA ELISA Kit; IL1F8 (Canonical product IL 1F8a) ELISA Kit; IL1F8 ELISA Kit; IL1F8_HUMAN ELISA Kit; IL1H2 ELISA Kit; IL36 beta ELISA Kit; IL36B ELISA Kit; Interleukin 1 family, member 8 (eta) ELISA Kit; Interleukin 1 family, member 8 ELISA Kit; Interleukin 1 homolog 2 ELISA Kit; Interleukin 1 Superfamily e ELISA Kit; Interleukin 36 beta ELISA Kit; Interleukin-1 eta ELISA Kit; Interleukin-1 family member 8 ELISA Kit; Interleukin-1 homolog 2 ELISA Kit; MGC126880 ELISA Kit; MGC126882 ELISA Kit; OTTMUSP00000012797 ELISA Kit; RP23-176J12.1 ELISA Kit
缩写：IL1F8
Uniprot No.：Q9NZH7
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 84-95 | |||||
回收率：
| The recovery of human IL1F8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 80-92 | ||||
| EDTA plasma (n=4) | 100 | 96-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.5372.5982.5682.404 5002.0622.0912.0771.913 2501.3991.3681.3841.220 1250.7990.7710.7850.621 62.50.5550.5340.5450.381 31.20.3740.3950.3850.221 15.60.2710.2940.2830.119 00.1630.1650.164 | ||||||
货期：3-5 working days
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