首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human soluble interleukin-1 receptor Ⅰ,IL-1sRⅠ ELISA kit

  • 中文名: 人白介素1可溶性受体Ⅰ(IL-1sRⅠ)ELISA Kit
货号: IPD-E04611h
Price: ¥2500
数量:
大包装询价

产品详情

  • 产品描述:
    人白介素1可溶性受体Ⅰ(IL-1sRⅠ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆,组织匀浆样本中的IL1R1含量。IL1R1是重要的靶点。白细胞介素 -1(IL -1)与其结合后参与免疫应答与炎症反应。相关研究围绕其信号通路机制,旨在深入了解其在疾病发生发展中的作用,为研发针对IL1R1的药物、改善炎症相关疾病治疗提供理论依据。试剂盒检测范围为0.156 ng/ml-10 ng/ml,灵敏度为0.039 ng/ml。适用于体外实验环境下对炎症机制、免疫调控通路及药物干预效果的科学研究,为研究IL - 1信号通路异常激活相关疾病的分子机制提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    IL1R1; IL1R; IL1RA; IL1RT1; Interleukin-1 receptor type 1; IL-1R-1; IL-1RT-1; IL-1RT1; CD121 antigen-like family member A; Interleukin-1 receptor alpha; IL-1R-alpha; Interleukin-1 receptor type I; p80; CD antigen CD121a
  • 缩写:
    IL1R1
  • Uniprot No.:
    P14778
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma,tissue homogenates
  • 检测范围:
    0.156 ng/ml-10 ng/ml
  • 灵敏度:
    0.039 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1sRⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    105


    Range %

    100-111


    1:2

    Average %

    92


    Range %

    87-98


    1:4

    Average %

    99


    Range %

    92-106


    1:8

    Average %

    92


    Range %

    87-98


  • 回收率:

    The recovery of human IL-1sRⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    106

    99-109


    EDTA plasma (n=4)

    103

    98-108


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.657

    2.699

    2.678

    2.506


    5

    2.188

    2.200

    2.194

    2.022


    2.5

    1.469

    1.470

    1.470

    1.298


    1.25

    0.778

    0.823

    0.801

    0.629


    0.625

    0.426

    0.457

    0.442

    0.270


    0.312

    0.333

    0.334

    0.334

    0.162


    0.156

    0.255

    0.240

    0.248

    0.076


    0

    0.177

    0.167

    0.172




  • 货期:
    3-5 working days

客户数据及评论

折叠内容

大包装询价

×