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产品描述：猪白介素1受体拮抗剂(IL1Ra)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1RN含量。IL1RN是重要的靶点。它是白细胞介素-1受体拮抗剂，可与IL-1竞争性结合IL-1受体，抑制IL-1的促炎作用。在研究中，聚焦其在炎症性疾病如类风湿关节炎等发病机制中的作用，以探索新的治疗策略。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品广泛应用于科研领域，包括但不限于猪类炎症性疾病模型的机制研究、免疫调节药物开发中的靶标验证，以及感染或应激条件下宿主免疫反应的动态监测，为探索IL - 1信号通路在动物生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1RN ELISA kit; IRAP1Interleukin-1 receptor antagonist protein ELISA kit; IL-1RN ELISA kit; IL-1ra ELISA kit; IRAP ELISA kit; IL1 inhibitor ELISA kit
缩写：IL1RN
Uniprot No.：Q29056
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 106 | |||||
| Range % | 93-110 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 91-105 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 87-101 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 84-95 | ||||||
回收率：
| The recovery of pig IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 85-96 | |||||
| EDTA plasma (n=4) | 108 | 98-112 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.2432.1872.2152.03220001.4411.3661.4041.22110000.9860.9540.9700.7875000.6580.6470.6530.4702500.4220.4020.4120.2291250.3520.3430.3480.16562.50.2770.2630.2700.08700.1860.1800.183 | |||||||
货期：3-5 working days
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