Mouse Interleukin 2,IL-2 ELISA kit

大包装询价

货号: IPD-E04627m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠白介素2(IL-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL2含量。IL2即白细胞介素-2，是机体免疫网络中重要的细胞因子。它能促进T细胞增殖、激活NK细胞等，在机体免疫调节和抗肿瘤免疫中发挥关键作用。研究多聚焦其信号传导机制及与免疫细胞相互作用，以挖掘其治疗免疫相关疾病的潜力。试剂盒检测范围为31.25 pg/ml- 2000 pg/ml，灵敏度为7.8 pg/ml。适用于小鼠感染模型、免疫性疾病研究或药物干预实验中IL - 2动态变化的监测，为免疫调控机制探索、炎症反应评估及治疗性抗体开发等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il2 ELISA kit; Il-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
缩写：
IL2
Uniprot No.：
P04351
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/ml - 2000 pg/ml
灵敏度：
7.8 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	95
	Range %	91-100
1:4	Average %	96
	Range %	90-101
1:8	Average %	92
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.151	2.234	2.192	2.078
1000	1.525	1.647	1.586	1.471
500	1.085	1.028	1.057	0.942
250	0.718	0.729	0.723	0.609
125	0.436	0.421	0.428	0.314
62.5	0.298	0.283	0.291	0.176
31.25	0.200	0.194	0.197	0.082
0	0.114	0.115	0.115

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse IL-2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled IL-2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.