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产品描述：山羊白介素2(IL-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL2含量。IL2是重要的细胞因子，由T细胞分泌。其背景在于对免疫系统有关键调节作用。研究机制上，IL2能与特异性受体结合，激活T细胞和NK细胞等免疫细胞的增殖与分化，增强机体免疫功能，在肿瘤免疫治疗等方面是研究热点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域中对山羊免疫相关疾病的机制探索、炎症模型分析或疫苗开发中细胞免疫应答的定量评估；为兽医研究、比较免疫学及畜牧领域中的免疫调控研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
缩写：IL2
Uniprot No.：P36835
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 94-107 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of goat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 95-102 | ||||
| EDTA plasma (n=4) | 93 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4912.3652.4282.3405001.5661.5941.5801.4922501.0301.0961.0630.9751250.6970.7530.7250.63762.50.4230.4430.4330.34531.20.2560.2770.2670.17915.60.1680.1690.1690.08100.0870.0890.088 | ||||||
货期：3-5 working days
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