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产品描述：仓鼠白介素1β (IL-1β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL1B含量。IL1B 即白介素-1β，是一种促炎细胞因子。它在炎症反应、免疫调节等生理和病理过程中扮演重要角色。研究表明，IL1B 可激活下游信号通路，促进炎症因子释放，与多种炎症性疾病、自身免疫性疾病的发生发展密切相关，是潜在的治疗靶点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于体外炎症模型构建、免疫调控机制研究以及生物制剂或天然产物的抗炎效果评价等科研场景，为探索IL - 1β在仓鼠疾病模型中的生物学功能提供可靠工具。其IL - 1β动态变化常用于评估感染性疾病模型、自身免疫机制及炎症相关药物干预研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-1β
Uniprot No.：Q9WVQ9
种属：Mesocricetus auratus (Golden hamster)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 105 | ||||
| Range % | 100-112 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of hamster IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.5782.6272.6032.4604002.0092.1062.0581.9152001.2271.2671.2471.1041000.5710.5350.5530.410500.3080.3290.3190.176250.2310.2190.2250.08212.50.2040.2150.2100.06700.1450.1400.143 | ||||||
货期：3-5 working days
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