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产品描述：牛白介素2(IL-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL2含量。IL2是重要的细胞因子，可调节免疫细胞生长、增殖和分化。它在免疫系统激活和维持免疫平衡方面作用关键。其研究机制主要围绕调控T细胞和NK细胞功能，在肿瘤免疫治疗、自身免疫性疾病等领域是研究热门靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研领域探究牛类免疫机制、疫苗效力评估、畜牧病原感染模型中的细胞免疫应答分析，以及炎症性疾病或免疫抑制相关研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
缩写：IL2
Uniprot No.：P05016
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 98-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1512.0992.1252.04010001.6831.6561.6701.5855001.2841.2701.2771.1922500.8460.8330.8400.7551250.5020.4980.5000.41562.50.3670.3560.3620.27731.250.2210.2130.2170.13200.0860.0840.085 | ||||||
货期：3-5 working days
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