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产品描述：犬白介素2(IL-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL2含量。IL2是重要的免疫细胞因子，其背景在于它能促进T细胞等免疫细胞生长和分化。研究机制上，IL2与受体结合，激活下游信号通路，调控免疫细胞功能，增强机体免疫应答，在肿瘤免疫治疗、自身免疫病研究等领域都是关键靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于兽医科研领域对犬类免疫相关疾病的机制研究，如感染性疾病模型建立、疫苗免疫效果评估、炎症反应动态监测等实验场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
缩写：IL2
Uniprot No.：Q29416
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 81-89 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 92-98 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-90 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 94-100 | ||||||
回收率：
| The recovery of dog IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 82-90 | |||||
| EDTA plasma (n=4) | 105 | 101-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.9192.9002.9102.8125002.4242.4682.4462.3482501.6701.6201.6451.5471250.9810.9670.9740.87662.50.4740.4810.4780.38031.20.2890.2760.2830.18515.60.1610.1630.1620.06400.0990.0970.098 | |||||||
货期：3-5 working days
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