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产品描述：猪白介素2(IL-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL2含量。IL2是重要的免疫细胞因子，在免疫调节中发挥关键作用。其背景在于它能促进T细胞、NK细胞等免疫细胞增殖与活化。研究机制上，IL2通过与受体结合激活下游信号通路，增强机体免疫功能，在肿瘤免疫治疗等领域研究广泛。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域中对猪类免疫系统功能的评估，例如疫苗研发中免疫激活效果分析、传染病模型中细胞免疫反应监测，或通过检测组织内IL - 2表达水平探究炎症性疾病的分子机制。为畜牧兽医研究、比较免疫学及动物疾病模型构建提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
缩写：IL2
Uniprot No.：P26891
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 80-99 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 82-91 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-102 | ||||
| EDTA plasma (n=4) | 96 | 91-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.8842.9152.9002.7645002.1232.1042.1141.9782501.2491.2721.2611.1251250.8180.8460.8320.69662.50.4920.4400.4660.33031.20.3140.3220.3180.18215.60.2630.2580.2610.12500.1350.1360.136 | ||||||
货期：3-5 working days
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