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产品描述：鸭白介素2(IL-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-2含量。IL-2是重要的免疫细胞因子，其背景在于在免疫调节中扮演关键角色。研究机制上，IL-2能刺激T细胞和NK细胞的生长、增殖与活化，增强机体免疫功能，在肿瘤免疫治疗、自身免疫病等研究领域备受关注，为相关疾病治疗带来新希望。试剂盒检测范围为6.25 pg/mL-400 pg/mL，操作流程标准化，支持科研人员高效开展鸭免疫相关研究，可用于评估病原感染后免疫细胞活性、炎症反应动态监测或免疫调节药物作用效果分析等实验场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-2
Uniprot No.：Q804B7
种属：Duck
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of duck IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 83-105 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 86-104 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 81-98 | |||||
回收率：
| The recovery of duck IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 85-105 | ||||
| EDTA plasma (n=4) | 94 | 89-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.3362.3422.3392.2012001.7871.7731.7801.6421001.3191.3061.3131.175500.8910.8980.8950.757250.6420.6510.6470.50912.50.4680.4940.4810.3436.250.2680.2530.2610.12300.1380.1370.138 | ||||||
货期：3-5 working days
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