Mouse Interleukin 21(IL-21) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E13162m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠白介素21(IL-21)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL21含量。IL-21是一种细胞因子。其它在免疫调节中发挥重要作用。研究机制上，IL-21可作用于多种免疫细胞，如T细胞、B细胞和NK细胞等，影响它们的增殖、分化和功能，在抗肿瘤免疫、自身免疫病等研究中是重要靶点。试剂盒检测范围为62.5 pg/ml-4000 pg/ml.，灵敏度为15.6 pg/ml.。适用于体外科研场景中免疫相关疾病模型的机制探索，如通过检测实验动物体液或组织样本的IL-21动态变化，评估炎症反应程度、疫苗免疫效果或免疫治疗干预后的生物标志物表达特征。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il21Interleukin-21 ELISA kit; IL-21 ELISA kit
缩写：
IL21
Uniprot No.：
Q9ES17
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
62.5 pg/ml-4000 pg/ml.
灵敏度：
15.6 pg/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-101
1:2	Average %	98
	Range %	92-103
1:4	Average %	103
	Range %	98-108
1:8	Average %	94
	Range %	88-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
4000	2.760	2.713	2.737	2.595
2000	2.431	2.333	2.382	2.240
1000	1.938	1.912	1.925	1.783
500	1.382	1.343	1.363	1.221
250	0.801	0.767	0.784	0.642
125	0.463	0.438	0.451	0.309
62.5	0.263	0.247	0.255	0.113
0	0.143	0.141	0.142