首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素23A(IL-23A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的IL23A含量。IL23A是一种编码白细胞介素23亚基的基因，与IL12B结合形成IL-23细胞因子，参与先天和适应性免疫。研究显示，IL23A在慢性炎症和肿瘤微环境中发挥重要作用，其可能通过促进Th17细胞分化、上调MMP-9表达等机制，影响肿瘤细胞的迁移和侵袭。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，本产品为科研人员研究IL - 23A在炎症反应、自身免疫调节中的分子机制提供可靠工具，也可用于体外药物开发过程中细胞因子表达水平的动态监测，为免疫相关疾病的基础研究和生物制剂的功效评估提供技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL 23 A ELISA Kit; IL 23 ELISA Kit; IL 23 subunit alpha ELISA Kit; IL 23A ELISA Kit; IL 23p19 ELISA Kit; IL-23 subunit alpha ELISA Kit; IL-23-A ELISA Kit; IL-23p19 ELISA Kit; IL12B ELISA Kit; IL23 ELISA Kit; Il23a ELISA Kit; IL23A_HUMAN ELISA Kit; IL23P19 ELISA Kit; interleukin 12B ELISA Kit; Interleukin 23 alpha subunit p19 ELISA Kit; Interleukin 23 p19 subunit ELISA Kit; interleukin 23 subunit alpha ELISA Kit; interleukin 23 subunit p19 ELISA Kit; interleukin six; G CSF related factor ELISA Kit; Interleukin-23 subunit alpha ELISA Kit; Interleukin-23 subunit p19 ELISA Kit; JKA3 induced upon T cell activation ELISA Kit; MGC79388 ELISA Kit; P19 ELISA Kit; SGRF ELISA Kit
缩写：IL23A
Uniprot No.：Q9NPF7
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 83-97 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-104 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 81-88 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 81-96 | ||||||
回收率：
| The recovery of human IL-23A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 94-101 | |||||
| EDTA plasma (n=4) | 107 | 100-114 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40001.7621.8451.8041.62820001.0011.0221.0120.83610000.6690.6670.6680.4925000.4070.4160.4120.2362500.2970.2770.2870.1111250.2530.2460.2500.07462.50.2200.2160.2180.04200.1720.1790.176 | |||||||
货期：3-5 working days
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