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产品描述：猪白介素-23 α亚基(IL23A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL23A含量。IL23A是重要的药物靶点。IL-23由IL23A和IL-12p40亚基组成，在自身免疫疾病病理中发挥关键作用。研究机制主要聚焦于其信号通路，通过阻断它与受体结合，可调节免疫反应，为相关疾病如银屑病等的治疗提供方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研领域对猪免疫系统功能的研究，如感染性疾病模型建立、免疫调节机制解析或炎症相关分子通路的探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL23A; SGRF; Interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; Interleukin-23 subunit p19; IL-23p19
缩写：IL23A
Uniprot No.：Q9N2H9
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL23A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of pig IL23A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 94 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5492.5982.5742.40410001.7891.7481.7691.5995001.0241.0421.0330.8632500.6030.6320.6180.4481250.4100.4160.4130.24362.50.3180.3380.3280.15831.250.2540.2650.2600.09000.1710.1690.170 | ||||||
货期：3-5 working days
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