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产品描述：人白介素26(IL-26)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆、细胞裂解物、组织培养上清液样本中的IL26含量。IL26是一种白细胞介素，属于IL-10家族，最初在疱疹病毒转化的T细胞中高表达。它可能参与黏膜免疫的局部机制，具有促炎功能。IL26可激活STAT1、STAT3、MAPK1/3（ERK1/2）、JUN和AKT，诱导SOCS3、TNF-α和IL-8的表达，并促进IL-8和IL-10的分泌及ICAM1的表面表达。IL26在多种炎症性疾病中发挥作用，其具体作用机制尚在研究中。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于基础科研中IL - 26蛋白表达的动态监测，包括但不限于炎症性疾病模型构建、病原体感染免疫应答研究、肿瘤微环境分析等方向，为探索IL - 26在免疫调控、组织修复及疾病进展中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AK155 ELISA Kit; IL 26 ELISA Kit; IL-26 ELISA Kit; IL26 ELISA Kit; IL26_HUMAN ELISA Kit; Interleukin 26 ELISA Kit; Interleukin-26 ELISA Kit; Protein AK155 ELISA Kit
缩写：IL26
Uniprot No.：Q9NPH9
种属：Homo sapiens (Human)
样本类型：serum, urine, tissue homogenates, cell lysates, cell culture supernates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-26 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of human IL-26 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 93-96 | ||||
| EDTA plasma (n=4) | 102 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.7492.7982.7742.616 20002.3152.3642.3402.182 10001.8431.8891.8661.708 5001.3991.3811.3901.232 2500.8990.8880.8940.736 1250.4830.4870.4850.327 62.50.3160.3290.3230.165 00.1570.1590.158 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-26 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled IL-26 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Squirt bottle, manifold dispenser, or automated microplate washer.
Absorbent paper for blotting the microtiter plate.
100ml and 500ml graduated cylinders.
Deionized or distilled water.
货期：3-5 working days
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