首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素25(IL-25)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清cellculturesupernates、尿液、组织匀浆样本中的IL25含量。IL25是一个值得关注的靶点。它属IL-17细胞因子家族成员，参与多种生理病理过程。研究机制上，IL25能激活相关信号通路，调节免疫细胞功能，在哮喘、过敏性疾病等免疫相关病症的发生发展中发挥作用。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于基础医学研究中炎症反应机制探索、免疫调控模型构建以及疾病生物标志物筛选等场景，例如通过血清样本分析体内IL - 25动态变化，或结合细胞培养实验评估体外分泌水平，为科研人员开展免疫相关疾病机制研究提供标准化检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL 17E ELISA Kit; IL 25 ELISA Kit; IL-17E ELISA Kit; IL-25 ELISA Kit; IL17e ELISA Kit; IL25 ELISA Kit; IL25_HUMAN ELISA Kit; Interleukin 17E ELISA Kit; Interleukin 25 ELISA Kit; Interleukin-17E ELISA Kit; Interleukin-25 ELISA Kit; Interleukin17E ELISA Kit; Interleukin25 ELISA Kit; OTTHUMP00000027946 ELISA Kit; OTTHUMP00000246238 ELISA Kit; UNQ3120/PRO10272 ELISA Kit
缩写：IL25
Uniprot No.：Q9H293
种属：Homo sapiens (Human)
样本类型：serum, cell culturesupernates, urine, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-103 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-106 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-103 | |||||
回收率：
| The recovery of human IL-25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.8922.7522.8222.71120002.2992.1792.2392.12810001.6451.5991.6221.5115000.9821.0020.9920.8812500.5610.6020.5820.4711250.3500.3660.3580.24762.50.2680.2750.2720.16100.1090.1120.111 | ||||||
货期：3-5 working days
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