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Human Interleukin 28A,IL-28A/IFN-λ2 ELISA kit

  • 中文名： 人白介素28A(IL-28A/IFN-λ2)ELISA Kit
货号: IPD-E11708h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人白介素28A(IL-28A/IFN-λ2)酶联免疫试剂盒，双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-28A/IFN-λ2含量。IL-28A/IFN-λ2作为靶点，属于Ⅲ型干扰素家族成员。其在抗病毒、抗肿瘤及免疫调节方面有潜在作用。研究机制上，它能与特异性受体结合，激活下游信号通路，调节相关基因表达，以发挥生物学效应，在医药研发领域受关注。试剂盒检测范围为0.156 ng/ml- 10 ng/ml，灵敏度为0.039 ng/ml。可满足科研领域对IL - 28A蛋白表达动态监测的需求，特别适用于病毒感染机制研究、免疫调控通路分析以及炎症相关疾病模型的实验探索，为免疫学研究、分子病理学实验及细胞因子网络分析提供可靠的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Cytokine ZCYTO20 ELISA Kit; IFN-lambda-2 ELISA Kit; IFNL2 ELISA Kit; IL-28A ELISA Kit; IL28A ELISA Kit; IL28A_HUMAN ELISA Kit; Interferon lambda 2 ELISA Kit; Interferon lambda-2 ELISA Kit; interleukin 28A (interferon, lambda 2) ELISA Kit; Interleukin 28A ELISA Kit; Interleukin-28A ELISA Kit; ZCYTO20 ELISA Kit
  • 缩写：
    IL-28A/IFN-λ2
  • Uniprot No.：
    Q8IZJ0
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/ml - 10 ng/ml
  • 灵敏度：
    0.039 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-28A/IFN-λ2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    104


    Range %

    100-108


    1:2

    Average %

    95


    Range %

    90-100


    1:4

    Average %

    90


    Range %

    85-94


    1:8

    Average %

    98


    Range %

    94-102


  • 回收率：

    The recovery of human IL-28A/IFN-λ2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    89

    84-93


    EDTA plasma (n=4)

    92

    88-96


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.815

    2.742

    2.779

    2.680


    5

    2.352

    2.196

    2.274

    2.175


    2.5

    1.673

    1.609

    1.641

    1.542


    1.25

    0.961

    1.004

    0.983

    0.884


    0.625

    0.527

    0.561

    0.544

    0.445


    0.312

    0.289

    0.295

    0.292

    0.193


    0.156

    0.204

    0.213

    0.209

    0.110


    0

    0.096

    0.101

    0.099



  • 货期：
    3-5 working days

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