首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素27(IL-27)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL27含量。IL-27是一种由p28和EBI3两个亚基组成的细胞因子，主要来源于活化的抗原提呈细胞，广泛分布于免疫细胞表面。它通过调节T细胞亚群的分化、增强自然杀伤细胞的作用以及抑制血管生成等机制，在抗肿瘤免疫中发挥作用。研究显示，IL-27可促进Th1细胞分化，抑制Th2和Th17细胞，并增强抗肿瘤免疫。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于探索炎症机制、评估药物干预效果或构建免疫调控模型等科研场景，为研究Th17/Treg细胞平衡、肿瘤微环境调控等课题提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il27 ELISA Kit; Il27aInterleukin-27 subunit alpha ELISA Kit; IL-27 subunit alpha ELISA Kit; IL-27-A ELISA Kit; IL27-A ELISA Kit; p28 ELISA Kit
缩写：IL27
Uniprot No.：Q8K3I6
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:2 | Average % | 106 | |||||
| Range % | 103-109 | ||||||
| 1:4 | Average % | 83 | |||||
| Range % | 80-85 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of mouse IL-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 95-105 | |||||
| EDTA plasma (n=4) | 94 | 89-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.5842.6312.6082.47110001.9791.9981.9891.8525001.3321.3011.3171.1802500.7820.7740.7780.6411250.4850.4680.4770.34062.50.2630.2780.2710.13431.250.2060.2140.2100.07300.1380.1350.137 | |||||||
货期：3-5 working days
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