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Human Interleukin 27,IL-27 ELISA Kit

  • 中文名： 人白介素27(IL-27)ELISA Kit
货号: IPD-E08464h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人白介素27(IL-27)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL27含量。IL27 是一个重要靶点。它属于细胞因子家族成员，在免疫调节方面背景意义深远。研究机制上，IL27 可通过与受体结合激活下游信号通路，调节 T 细胞等免疫细胞功能，参与炎症反应与肿瘤免疫等过程，为相关疾病治疗提供思路。试剂盒检测范围为0.156 ng/ml- 10 ng/ml，灵敏度为0.039 ng/ml。科研人员可通过该产品定量分析IL-27在炎症模型、免疫调控实验或疾病机制研究中的动态变化，为探索免疫调节通路、评估药物干预效果或生物标志物筛选提供可靠数据支持；适用于分子生物学、免疫学及相关领域的体外科研实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    EBI3 ELISA Kit; IL 27 ELISA Kit; IL 27 p28 subunit ELISA Kit; IL 27A ELISA Kit; IL 30 ELISA Kit; IL-27 subunit alpha ELISA Kit; IL-27-A ELISA Kit; IL27 ELISA Kit; IL27-A ELISA Kit; IL27A ELISA Kit; IL27A_HUMAN ELISA Kit; IL27p28 ELISA Kit; IL30 ELISA Kit; Interleukin 27 ELISA Kit; Interleukin 27 subunit alpha ELISA Kit; Interleukin 27; 28-KD subunit ELISA Kit; Interleukin 30 ELISA Kit; Interleukin-27 subunit alpha ELISA Kit; p28 ELISA Kit
  • 缩写：
    IL27
  • Uniprot No.：
    Q8NEV9
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/ml - 10 ng/ml
  • 灵敏度：
    0.039 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    90-98


    1:2

    Average %

    89


    Range %

    84-95


    1:4

    Average %

    92


    Range %

    87-96


    1:8

    Average %

    97


    Range %

    92-104


  • 回收率：

    The recovery of human IL-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    88

    84-93


    EDTA plasma (n=4)

    90

    88-93


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.815

    2.869

    2.842

    2.717


    5

    2.436

    2.647

    2.542

    2.417


    2.5

    1.954

    1.968

    1.961

    1.836


    1.25

    1.312

    1.336

    1.324

    1.199


    0.625

    0.764

    0.704

    0.734

    0.609


    0.312

    0.468

    0.442

    0.455

    0.330


    0.156

    0.297

    0.274

    0.286

    0.161


    0

    0.124

    0.126

    0.125




  • 货期：
    3-5 working days

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