Mouse soluble interleukin-2 receptor,sIL-2Rα ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E07440m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠白介素2可溶性受体α链(sIL-2Rα/CD25)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL2RA含量。IL2RA，即白介素-2受体α亚基，是一种细胞表面蛋白质，由人类基因IL2RA编码，与β亚基和γ亚基共同组成白介素-2受体。它在免疫系统中发挥重要作用，参与调节T细胞增殖和免疫反应，是免疫治疗和免疫调节的关键靶点。试剂盒检测范围为15.6 pg/ml- 1000 pg/ml，灵敏度为3.9 pg/ml。在免疫学研究、疾病模型构建及药物作用机制探索中具有重要应用价值，例如用于评估实验动物免疫系统激活状态、监测炎症相关疾病进展或验证免疫调节剂对sIL-2Rα分泌的影响，为科研人员提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il2ra; Il2r; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; CD antigen CD25
缩写：
IL2RA
Uniprot No.：
P01590
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/ml - 1000 pg/ml
灵敏度：
3.9 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse sIL-2Rα/CD25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	108
	Range %	104-112
1:4	Average %	89
	Range %	85-93
1:8	Average %	107
	Range %	103-110

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	2.813	2.690	2.752	2.575
500	2.221	2.301	2.261	2.084
250	1.583	1.601	1.592	1.415
125	0.945	0.931	0.938	0.761
62.5	0.626	0.601	0.614	0.437
31.2	0.387	0.361	0.374	0.197
15.6	0.303	0.302	0.303	0.126
0	0.176	0.178	0.177