Human Interleukin 31(IL-31) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E16999h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人白介素31(IL-31)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的IL31含量。IL31是一个受关注的靶点。它是一种细胞因子，与皮肤瘙痒及炎症反应密切相关。研究机制主要聚焦在其与受体结合后激活下游信号通路，引发免疫反应和神经传导改变，目前围绕它在皮肤病治疗等领域开展诸多研究。试剂盒检测范围为125 pg/ml-8000 pg/ml.，灵敏度为31.25 pg/ml.。适用于体外研究中对IL-31表达水平的动态监测，如炎症性疾病机制探索、免疫调节药物开发或皮肤病理模型评估等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
IL 31 ELISA Kit; IL-31 ELISA Kit; IL31 ELISA Kit; IL31_HUMAN ELISA Kit; Interleukin 31 ELISA Kit; Interleukin-31 ELISA Kit
缩写：
IL31
Uniprot No.：
Q6EBC2
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
125 pg/ml-8000 pg/ml.
灵敏度：
31.25 pg/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-96
1:2	Average %	96
	Range %	90-102
1:4	Average %	84
	Range %	81-87
1:8	Average %	88
	Range %	83-94

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
8000	1.774	1.811	1.793	1.627
4000	1.003	1.055	1.029	0.863
2000	0.666	0.687	0.677	0.511
1000	0.401	0.412	0.407	0.241
500	0.289	0.302	0.296	0.130
250	0.245	0.255	0.250	0.084
125	0.211	0.216	0.214	0.048
0	0.163	0.168	0.166