首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素31(IL-31)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL31含量。IL31，即白介素31，是一种炎症性细胞因子，主要由Th2细胞产生，通过IL31RA和OSMR受体复合物激活STAT3和STAT5等信号通路，参与皮肤免疫和炎症性疾病，如特应性皮炎。其作用机制涉及皮肤屏障、神经生长和瘙痒调节。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研领域探究IL-31在炎症模型、免疫调控实验中的动态表达，或用于评估药物干预对IL-31分泌的影响，可为皮肤病学、免疫学等基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il31Interleukin-31 ELISA Kit; IL-31 ELISA Kit
缩写：IL31
Uniprot No.：Q6EAL8
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 82-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 87-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-100 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of mouse IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-104 | ||||
| EDTA plasma (n=4) | 99 | 90-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.8862.8462.8662.70210002.3472.3362.3422.1785001.5591.6021.5811.4172500.8970.8780.8880.7241250.5320.5570.5450.38162.50.3660.3740.3700.20631.250.2620.2450.2540.09000.1620.1650.164 | ||||||
货期：3-5 working days
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