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产品描述：人白介素32(IL-32)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL32含量。IL32是一个备受关注的靶点。它在免疫调节等生理过程中发挥重要作用。相关研究围绕其在疾病发生发展中的机制展开，探究其如何参与炎症反应、细胞信号传导等。明确IL32的作用机制，有望为相关疾病的治疗提供新的靶点和思路。试剂盒检测范围为312.5 pg/mL-20000 pg/mL，该产品适用于基础医学研究、炎症相关信号通路探索、疾病动物模型评估以及药物研发过程中生物标志物检测等科研场景，为体外实验提供高效可靠的检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL 32alpha ELISA Kit; IL 32beta ELISA Kit; IL 32delta ELISA Kit; IL 32gamma ELISA Kit; IL-32 ELISA Kit; IL32 ELISA Kit; IL32_HUMAN ELISA Kit; Interleukin 32 ELISA Kit; Interleukin 32 small ELISA Kit; Interleukin 32 theta ELISA Kit; Interleukin-32 ELISA Kit; interleukin-32 eta ELISA Kit; Natural killer cell transcript 4 ELISA Kit; Natural killer cells protein 4 ELISA Kit; NK 4 ELISA Kit; NK4 ELISA Kit; OTTHUMP00000236040 ELISA Kit; OTTHUMP00000236041 ELISA Kit; OTTHUMP00000236042 ELISA Kit; OTTHUMP00000236043 ELISA Kit; OTTHUMP00000236044 ELISA Kit; OTTHUMP00000236045 ELISA Kit; OTTHUMP00000236046 ELISA Kit; OTTHUMP00000236047 ELISA Kit; OTTHUMP00000236048 ELISA Kit; OTTHUMP00000236049 ELISA Kit; OTTHUMP00000236050 ELISA Kit; OTTHUMP00000236051 ELISA Kit; OTTHUMP00000236052 ELISA Kit; OTTHUMP00000236053 ELISA Kit; OTTHUMP00000236054 ELISA Kit; OTTHUMP00000241545 ELISA Kit; OTTHUMP00000241602 ELISA Kit; OTTHUMP00000241603 ELISA Kit; OTTHUMP00000241604 ELISA Kit; OTTHUMP00000241605 ELISA Kit; OTTHUMP00000241606 ELISA Kit; OTTHUMP00000241607 ELISA Kit; OTTHUMP00000241608 ELISA Kit; OTTHUMP00000241609 ELISA Kit; OTTHUMP00000241610 ELISA Kit; OTTHUMP00000241611 ELISA Kit; OTTHUMP00000241612 ELISA Kit; TAIF ELISA Kit; TAIFa ELISA Kit; TAIFb ELISA Kit; TAIFc ELISA Kit; TAIFd ELISA Kit; Tumor necrosis factor alpha-inducing factor ELISA Kit
缩写：IL32
Uniprot No.：P24001
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：312.5 pg/mL-20000 pg/mL
灵敏度：78.125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-32 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 89 | |
| Range % | 82-97 | ||
| 1:2 | Average % | 98 | |
| Range % | 94-104 | ||
| 1:4 | Average % | 97 | |
| Range % | 90-105 | ||
| 1:8 | Average % | 87 | |
| Range % | 82-92 |
回收率：
| The recovery of human GROα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 101 | 96-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 200002.8012.7522.7772.627 100002.4402.2792.3602.210 50001.7841.7891.7871.637 25000.9611.0020.9820.832 12500.5470.5750.5610.411 6250.3380.3460.3420.192 312.50.2340.2250.2300.080 00.1460.1540.150 | ||||||
货期：3-5 working days
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