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产品描述：犬白介素33(IL33)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL33含量。IL33是重要靶点，其作为白细胞介素家族成员，在免疫调节中起关键作用。研究发现它能激活下游信号通路，调控免疫细胞功能。异常表达与哮喘、炎症性肠病等多种炎症疾病相关，对其深入研究有助于开发新的免疫治疗策略。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，本试剂盒可用于探索犬类疾病模型中的免疫调控机制、炎症相关研究或药物干预实验中的生物标志物检测。其操作流程兼容常规实验室条件，可为兽医基础研究、比较医学或实验动物模型开发提供可靠的数据支持，帮助科研人员深入解析IL33在犬类免疫系统中的功能及调控网络。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL33Interleukin-33 ELISA Kit; IL-33 ELISA Kit; Protein DVS27 ELISA Kit
缩写：IL33
Uniprot No.：O97863
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 82-89 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 93-90 | ||||||
回收率：
| The recovery of dog IL33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 88-95 | |||||
| EDTA plasma (n=4) | 97 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.9292.9682.9492.8537502.4062.3002.3532.2573751.6311.6451.6381.542187.50.9150.9350.9250.82993.750.5060.5000.5030.40746.880.2540.2750.2650.16923.440.1410.1520.1470.05100.0940.0980.096 | |||||||
货期：3-5 working days
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