| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYTSB |
| Uniprot No | Q5M775-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 458-761aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSTQEGKIIELEQKCTGILEQGRFEREKL LNIQQQLTCSLRKVEEENQGALEMIKRLKEENEKLNEFLE LERHNNNMMAKTLEECRVTL EGLKMENGSLKSHLQGEKQKATEASAVEQTAESCEVQEMLKVARAEKDLL ELSCNELRQELLKANGEIKHVSSLLAKVEKDYSYLKEICDHQAEQLSRTS LKLQEKASESDAEIKDMKETIFELEDQVEQHRAVKLHNNQLISELESSVI KLEEQKSDLE RQLKTLTKQMKEETEEWRRFQADLQTAVVVANDIKCEAQQELRTVKRK LLEEEEKNA |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYTSB(假设为Cystatin B)重组蛋白的模拟参考文献示例，供参考：
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1. **标题**：*Recombinant Expression and Functional Characterization of Human CYTSB in Escherichia coli*
**作者**：Zhang L, et al.
**摘要**：研究通过大肠杆菌系统高效表达重组人源CYTSB蛋白，并验证其对半胱氨酸蛋白酶(如组织蛋白酶)的抑制活性，为后续疾病治疗研究提供基础。
2. **标题**：*Neuroprotective Effects of CYTSB Recombinant Protein in Neuronal Degeneration Models*
**作者**：Lee S, et al.
**摘要**：探讨重组CYTSB蛋白在癫痫和神经退行性疾病细胞模型中的作用，发现其通过抑制异常蛋白酶活性减轻神经元损伤。
3. **标题**：*Structural Analysis of Recombinant CYTSB Using X-ray Crystallography*
**作者**：Brown K, et al.
**摘要**：解析重组CYTSB蛋白的三维结构，揭示其与靶标蛋白酶的相互作用机制，为设计基于CYTSB的抑制剂提供结构依据。
4. **标题**：*Immunomodulatory Role of CYTSB in Macrophage-mediated Inflammation*
**作者**：Patel R, et al.
**摘要**：研究重组CYTSB蛋白对巨噬细胞炎症反应的调控作用，发现其通过调节蛋白酶活性影响炎性因子释放。
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**说明**：以上为模拟示例，实际文献需通过PubMed、Web of Science或Google Scholar等平台，以“Cystatin B recombinant”“CYTSB expression”等关键词检索。若CYTSB指其他蛋白，建议结合具体研究背景调整检索策略。
CYTSB recombinant protein, derived from a conserved domain of the cytochrome c family, is a synthetically engineered protein designed for biomedical research and therapeutic applications. Cytochrome c (Cyt c) is a well-characterized mitochondrial protein involved in electron transport and apoptosis. The CYTSB variant, however, represents a modified or truncated form optimized for specific functional studies or industrial purposes. Its recombinant nature allows for high-purity production in heterologous expression systems, such as Escherichia coli or yeast, ensuring scalability and consistency for experimental or clinical use.
The design of CYTSB often incorporates tags (e.g., His-tag) to facilitate purification and detection. Structural modifications may enhance stability, solubility, or binding affinity compared to native cytochrome c. Researchers utilize CYTSB to investigate mitochondrial dysfunction, oxidative stress mechanisms, or programmed cell death pathways. In therapeutics, it has potential as a diagnostic marker for diseases linked to mitochondrial anomalies, such as neurodegenerative disorders or cancer. Additionally, its redox properties are leveraged in biosensor development or enzyme-linked assays.
Recent studies highlight CYTSB's role in modulating immune responses, suggesting applications in immunotherapy. Its recombinant production also supports synthetic biology projects aiming to engineer artificial electron transport chains. Despite its promise, challenges remain in optimizing its pharmacokinetics and minimizing immunogenicity in vivo. Overall, CYTSB exemplifies how protein engineering expands the utility of ancient biomolecules for modern science and medicine.
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