| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD43 |
| Uniprot No | P16150 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-253aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSSTTAVQT PTSGEPLVST SEPLSSKMYT TSITSDPKAD STGDQTSALP PSTSINEGSP LWTSIGASTG SPLPEPTTYQ EVSIKMSSVP QETPHATSHP AVPITANSLG SHTVTGGTIT TNSPETSSRT SGAPVTTAAS SLETSRGTSG PPLTMATVSL ETSKGTSGPP VTMATDSLET STGTTGPPVT MTTGSLEPSS GASGPQVSSV KLSTMMSPTT STNASTVPFR NPDENSR |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CD43重组蛋白的参考文献及其摘要概括：
1. **文献名称**: *"Expression and functional characterization of recombinant CD43 in T-cell activation"*
**作者**: Smith A, et al.
**摘要**: 研究报道了通过哺乳动物表达系统成功制备重组CD43蛋白，并证明其通过调控T细胞表面受体信号通路参与T细胞活化过程。
2. **文献名称**: *"Structural analysis of glycosylated CD43 recombinant protein reveals its role in leukocyte adhesion"*
**作者**: Tanaka K, et al.
**摘要**: 通过重组CD43的糖基化结构解析，揭示了其胞外域通过特定糖链修饰介导白细胞与内皮细胞的黏附作用。
3. **文献名称**: *"CD43 recombinant protein inhibits HIV-1 entry by blocking viral envelope glycoprotein interaction"*
**作者**: Lee H, et al.
**摘要**: 发现重组CD43蛋白可通过竞争性结合HIV-1包膜蛋白gp120.干扰病毒与宿主细胞膜融合，为抗病毒药物开发提供新策略。
备注：以上文献信息为示例性内容，实际引用时需核实具体文献来源及细节。
CD43. also known as leukosialin or sialophorin, is a heavily glycosylated transmembrane protein predominantly expressed on the surface of leukocytes, including T cells, neutrophils, and monocytes. It belongs to the sialomucin family, characterized by an extended extracellular domain rich in serine and threonine residues that serve as sites for O-linked glycosylation. This glycosylation contributes to its anti-adhesive properties, modulating cell-cell and cell-matrix interactions. CD43 plays multifaceted roles in immune regulation, including T-cell activation, apoptosis, and leukocyte migration. Dysregulation of CD43 has been implicated in immune disorders, hematologic malignancies, and inflammatory diseases.
Recombinant CD43 protein is engineered to study its structure-function relationships and therapeutic potential. Produced via heterologous expression systems (e.g., mammalian cells, bacteria, or insect cells), the recombinant form allows controlled investigation of CD43’s extracellular domain, which mediates most ligand interactions. Researchers often focus on its truncated or soluble variants to bypass membrane-associated complexities. The protein’s glycosylation pattern, critical for its biological activity, is typically preserved using mammalian expression systems like HEK293 or CHO cells.
CD43’s recombinant form has become a vital tool for developing antibodies, studying immune synapse formation, and exploring its dual role as both an adhesion inhibitor and a signaling molecule. Recent studies highlight its interaction with ligands like galectin-1 and E-selectin, offering insights into cancer metastasis and autoimmune therapies. Additionally, recombinant CD43 serves as a potential biomarker or therapeutic target in leukemia, where its aberrant expression correlates with disease progression. Ongoing research aims to harness its immunomodulatory properties for novel biologics, emphasizing its context-dependent roles in health and disease.
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