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产品描述：山羊白介素4(IL-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL4含量。IL4是重要的细胞因子。其背景上，它在免疫系统中起关键作用，参与免疫反应调节。研究机制方面，IL4能作用于多种免疫细胞，如T、B淋巴细胞等，可调节细胞的增殖、分化，还参与Th2细胞相关免疫应答过程，与过敏等疾病病理机制关联密切。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可应用于动物免疫学基础研究，如寄生虫感染模型中的Th2免疫应答评估、过敏性疾病机制探究或疫苗效价研究中的免疫指标分析，为科研人员解析山羊免疫调控网络提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写：IL4
Uniprot No.：P79155
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of goat IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7752.7992.7872.6455002.1091.9982.0541.9122501.3711.3871.3791.2371250.7910.7860.7890.64762.50.5050.5410.5230.38131.20.3570.3680.3630.22115.60.2780.2980.2880.14600.1410.1430.142 | ||||||
货期：3-5 working days
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