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产品描述：犬白介素4(IL-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL4含量。IL4是重要的细胞因子，在免疫应答等生理过程发挥关键作用。其研究机制主要围绕与IL4受体结合后激活下游信号通路，调节细胞增殖、分化等，在过敏、哮喘等疾病病理机制中有关键作用，也是药物研发热门靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于科研领域中对犬类免疫相关疾病机制的研究，例如过敏性疾病模型构建、寄生虫感染后免疫应答分析、疫苗效果评估或免疫调节药物开发等实验场景，为探索犬类免疫调控网络提供精准的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写：IL4
Uniprot No.：O77762
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of dog IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-105 | ||||
| EDTA plasma (n=4) | 92 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.9122.9862.9492.8532002.5462.5312.5392.4431001.7801.8311.8061.710501.1271.2071.1671.071250.5460.5880.5670.47112.50.2590.2760.2680.1726.250.1680.1760.1720.07600.0950.0960.096 | ||||||
货期：3-5 working days
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