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产品描述：绵羊白介素4(IL-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL4含量。IL4是一种由活化的T淋巴细胞、肥大细胞、嗜碱性粒细胞产生的多效性细胞因子，在免疫调节中起关键作用。它通过诱导B细胞分化、促进抗体产生和调节Th2细胞介导的免疫反应，在过敏性疾病和肿瘤免疫中扮演重要角色。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于免疫学研究、炎症性疾病模型分析、疫苗免疫效果评估等科研领域，可用于探究绵羊感染性疾病、寄生虫免疫应答及自身免疫机制，为其提供精准的蛋白定量数据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL4 ELISA Kit; IL-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写：IL4
Uniprot No.：P30368
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 93-100 | |||||
回收率：
| The recovery of sheep IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 96 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.0841.9582.0211.8692001.3001.2761.2881.1361000.8280.8170.8230.671500.5180.5290.5240.372250.3330.3430.3380.18612.50.2810.2900.2860.1346.250.2310.2240.2280.07600.1540.1500.152 | ||||||
货期：3-5 working days
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