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产品描述：牛白介素6(IL-6)酶联免疫试剂盒，竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL6含量。IL-6（白介素6）是一种多功能细胞因子，由多种细胞类型产生，如单核巨噬细胞、成纤维细胞、T淋巴细胞等。它通过与其受体结合，参与调节免疫反应、急性期反应和造血功能。IL-6在多种疾病的发生发展中扮演重要角色，如炎症、自身免疫疾病和癌症。其研究机制涉及IL-6与受体的相互作用、信号传导途径（如JAK2/STAT3）的激活，以及其在细胞增殖、分化和迁移中的作用。试剂盒检测范围为5 pg/mL-1000 pg/mL，适用于畜牧领域炎症性疾病研究、疫苗效力评估、抗感染免疫机制探索等科研场景，也可用于动物源性生物制品（如血清或细胞培养上清）的质量控制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL6 Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写：IL6
Uniprot No.：P26892
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：5 pg/mL-1000 pg/mL
灵敏度：2.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-101 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of bovine IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 94-101 | ||||
| EDTA plasma (n=4) | 96 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.0710.0750.073 2500.1920.2050.199 800.4210.4510.436 200.8760.8970.887 51.3451.3161.331 02.1162.2152.166 | ||||||
货期：3-5 working days
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