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产品描述：山羊白介素6(IL-6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL6含量。IL6（白细胞介素-6）是一种多功能的细胞因子，参与免疫调节、炎症反应、应激响应等生理过程。其研究机制主要涉及IL6与受体gp130结合，激活下游信号通路如JAK-STAT，调节细胞增殖、分化和功能。靶向IL6及其信号通路已成为治疗多种炎症和自身免疫性疾病的重要策略。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于实验室环境下山羊IL - 6蛋白的表达动力学研究、炎症模型评估、疫苗或药物干预效果分析等科研场景。为畜牧兽医研究、比较医学及动物免疫学实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写：IL6
Uniprot No.：Q28319
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-91 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 96-104 | |||||
回收率：
| The recovery of goat IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-101 | ||||
| EDTA plasma (n=4) | 102 | 97-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.6252.6982.6622.542501.9592.0011.9801.860251.2211.3141.2681.14812.50.7410.7050.7230.6036.250.4550.4680.4620.3423.120.3070.3180.3130.1931.560.2380.2370.2380.11800.1210.1190.120 | ||||||
货期：3-5 working days
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