关键信息
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基因名
Carbonic Anhydrase 4
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简介
Carbonic anhydrase 4 catalyzes the reversible hydration of carbon dioxide and plays a crucial role in maintaining intracellular and extracellular pH. Carbonic Anhydrase 4 Protein, Human (HEK293, His) is the recombinant human-derived Carbonic Anhydrase 4 protein, expressed by HEK293 , with C-His labeled tag.
- 应用
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别名
Carbonic anhydrase 4; Carbonate dehydratase IV; CA-IV; CA4
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种属
Human
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表达系统
HEK293
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标签
C-10*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P22748-1
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表达区间
A19-K283
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氨基酸序列
MRMLLALLALSAARPSASAESHWCYEVQAESSNYPCLVPVKWGGNCQKDRQSPINIVTTKAKVDKKLGRFFFSGYDKKQTWTVQNNGHSVMMLLENKASISGGGLPAPYQAKQLHLHWSDLPYKGSEHSLDGEHFAMEMHIVHEKEKGTSRNVKEAQDPEDEIAVLAFLVEAGTQVNEGFQPLVEALSNIPKPEMSTTMAESSLLDLLPKEEKLRHYFRYLGSLTTPTCDEKVVWTVFREPIQLHREQILAFSQKLYYDKEQTVSMKDNVRPLQQLGQRTVIK
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蛋白长度
Partial
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分子量
33 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Carbonic Anhydrase 4 (CA4) is an enzyme that plays a critical role in maintaining acid-base balance in various tissues, particularly in the lungs and kidneys. It catalyzes the reversible reaction between carbon dioxide and water to form bicarbonate and protons, thereby facilitating gas exchange and ion transport. The importance of CA4 in physiological processes has made it a target for research in various fields, including respiratory diseases, kidney disorders, and cancer. Mutations or dysregulation of CA4 can lead to pathological conditions, making it imperative to understand its structure and function. Recent advances in recombinant DNA technology have enabled the production of recombinant CA4, allowing for detailed studies of its enzymatic activity and interaction with inhibitors or substrates. This research enhances our understanding of CA4's role in health and disease, paving the way for potential therapeutic interventions. The study of recombinant CA4 not only contributes to basic biochemical knowledge but also holds promise for developing new strategies in treating conditions linked to acid-base imbalances and abnormal carbon dioxide metabolism.
