| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SRP14 |
| Uniprot No | P37108 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-160aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMVLLESEQFLTELTRLFQKCRTSGSV YITLKKYDGRTKPIPKKGTVEGFEPADNKCLLRATDGKKK ISTVVSSKEVNKFQMAYSNLLRANMDGLKKRDKKNKTKKTKAAAAAAAAA PAAAATAATTAATTAATAAQ |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SRP14重组蛋白的3篇参考文献示例(注：文献信息为模拟示例，实际引用请以真实文献为准)：
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1. **文献名称**: *"Recombinant expression and functional analysis of the SRP14 subunit in mammalian cells"*
**作者**: Müller, J., et al.
**摘要**: 本研究通过哺乳动物细胞表达系统成功重组表达了SRP14蛋白，并验证其与SRP复合体其他亚基(如SRP19和SRP54)的相互作用，证实SRP14在信号肽识别中的关键作用。
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2. **文献名称**: *"Structural insights into SRP14-mediated protein targeting in archaea"*
**作者**: Zhang, Y., & Li, R.
**摘要**: 通过重组表达古菌来源的SRP14蛋白，结合X射线晶体学分析其三维结构，揭示了SRP14与RNA结合域的相互作用机制，为原核生物蛋白质靶向机制提供新见解。
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3. **文献名称**: *"Development of a bacterial expression system for human SRP14 and its application in drug screening"*
**作者**: Chen, H., et al.
**摘要**: 报道了利用大肠杆菌系统高效表达人源SRP14重组蛋白的优化方案，并通过体外实验验证其结合小分子抑制剂的潜力，为靶向SRP的药物开发提供技术基础。
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如需具体文献，建议在PubMed或Google Scholar中检索关键词 **"SRP14 recombinant protein"** 或 **"SRP14 expression"**。
Signal Recognition Particle 14 kDa protein (SRP14) is a critical component of the signal recognition particle (SRP), a ribonucleoprotein complex essential for co-translational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER) in eukaryotes and archaea. The SRP comprises a noncoding RNA (SRP RNA) and six protein subunits (SRP9. SRP14. SRP19. SRP54. SRP68. SRP72) in mammals. SRP14 forms a heterodimer with SRP9. binding to the Alu domain of SRP RNA. This SRP9/14-RNA complex is responsible for transiently halting translation elongation, ensuring proper synchronization between protein synthesis and ER translocation.
Recombinant SRP14 is produced using genetic engineering techniques, typically by expressing the cloned SRP14 gene in bacterial (e.g., *E. coli*) or eukaryotic systems. Purification often involves affinity tags (e.g., His-tag) for efficient isolation. Recombinant SRP14 retains its ability to interact with SRP9 and SRP RNA, enabling functional studies of SRP assembly and its role in protein trafficking.
Research applications include elucidating SRP-mediated protein sorting mechanisms, investigating mutations linked to congenital disorders (e.g., SRP-dependent protein targeting deficiencies), and developing therapeutics targeting secretory pathway dysfunctions. Its recombinant form also facilitates structural studies (e.g., X-ray crystallography, cryo-EM) to resolve molecular interactions within the SRP complex. The protein's conserved nature across species underscores its fundamental role in cellular homeostasis.
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