首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子白介素8(IL-8/CXCL8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL8含量。CXCL8是一种趋化因子，在炎症反应、免疫调节等生理病理过程中发挥重要作用。其研究机制主要聚焦于与受体结合，介导细胞趋化和激活，可调节中性粒细胞迁移和活化，在感染、肿瘤、自身免疫病等疾病中表达常异常，是潜在治疗靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于实验室中炎症机制研究、免疫调控实验及药物开发过程中的生物标志物动态监测，例如在细菌感染模型、创伤修复研究或抗炎药物药效评估中量化IL - 8的表达变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant/activation protein 1 ELISA Kit; NAP-1 ELISA Kit; Permeability factor 1 ELISA Kit; PF1 ELISA Kit; rPF1 ELISA Kit
缩写：CXCL8
Uniprot No.：P19874
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 101 | ||||
| Range % | 94-105 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-96 | ||||
| EDTA plasma (n=4) | 92 | 89-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.3902.3452.3682.2857501.5871.4761.5321.4493750.8140.7780.7960.713187.50.4090.3890.3990.316940.2760.2640.2700.187470.1820.1720.1770.09423.50.1340.1310.1330.05000.0850.0810.083 | ||||||
货期：3-5 working days
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