首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素9(IL-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL9含量。IL9是一个重要的研究靶点。它是白细胞介素家族成员，在免疫调节等生理过程中发挥作用。研究机制方面，IL9可与相应受体结合，激活下游信号通路，参与多种疾病发生发展，如哮喘、自身免疫病等，研究其机制对疾病治疗有重要意义。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，科研人员可借助本产品探究IL - 9在疾病模型中的表达变化，例如过敏性疾病机制研究、免疫细胞功能调控或药物干预效果评估等方向，为免疫相关基础研究提供精准工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il9 ELISA Kit; Interleukin-9 ELISA Kit; IL-9 ELISA Kit; Cytokine P40 ELISA Kit; T-cell growth factor P40 ELISA Kit
缩写：IL9
Uniprot No.：P15247
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 105 | ||||
| Range % | 100-112 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-95 | |||||
回收率：
| The recovery of mouse IL-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 88-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4782.5202.4992.3935001.8941.8761.8851.7792501.2611.2041.2331.1271250.7740.8090.7920.68662.50.4960.5160.5060.40031.20.2940.2750.2850.17915.60.2050.2100.2080.10200.1050.1070.106 | ||||||
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