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产品描述：鱼白介素8(IL-8/CXCL8)酶联免疫试剂盒，定量检测血清、血浆样本中的IL8/CXCL8含量。IL8/CXCL8属于趋化因子家族，在多种生理和病理过程中发挥作用，如炎症反应、肿瘤发展等。研究发现其能招募免疫细胞到炎症部位，参与肿瘤血管生成、细胞增殖和转移等过程，是炎症相关疾病和肿瘤治疗研究的潜在靶点。试剂盒检测范围为31.25 pg/mL-500 pg/mL，满足实验室对鱼类疾病模型构建、免疫调节机制研究或环境胁迫响应分析的定量检测需求；支持科研人员高效开展水产动物免疫学、病理学等基础研究，为揭示鱼类先天免疫机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL8/CXCL8
Uniprot No.：Q6F4N2
种属：Fish
样本类型：serum, plasma
检测范围：31.25 pg/mL-500 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 92-97 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 85-100 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 89-101 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 86-100 | ||||||
回收率：
| The recovery of fish IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 89-100 | |||||
| EDTA plasma (n=4) | 95 | 85-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2Average 5000.1720.1760.174 2500.3920.3990.396 1250.6350.6280.632 62.51.0381.0461.042 31.252.4562.4172.437 | |||||||
货期：3-5 working days
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