首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：绵羊白介素8(IL-8/CXCL8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL8含量。CXCL8是重要的趋化因子，在炎症和免疫反应中发挥关键作用。其背景在于可吸引中性粒细胞至炎症部位。研究机制方面，主要是通过与相应受体结合，激活下游信号通路，影响细胞的迁移、增殖等行为，在感染、肿瘤等疾病研究中备受关注。试剂盒检测范围为6.25 pg/mL-400 pg/mL，本试剂盒适用于科研领域中对绵羊模型中炎症相关信号通路的机制研究、药物干预效果评估或疾病模型构建等实验需求，96孔板规格设计满足高通量样本分析要求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
缩写：CXCL8
Uniprot No.：P36925
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 85 | |||||||||||
| Range % | 80-90 | ||||||||||||
| 1:2 | Average % | 88 | |||||||||||
| Range % | 80-95 | ||||||||||||
| 1:4 | Average % | 94 | |||||||||||
| Range % | 87-99 | ||||||||||||
| 1:8 | Average % | 97 | |||||||||||
| Range % | 90-105 | ||||||||||||
回收率：
| The recovery of sheep IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 92 | 85-100 | |||||||||||
| EDTA plasma (n=4) | 96 | 91-103 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| pg/mlOD1OD2AverageCorrected4002.3122.2312.2722.0942001.7531.8541.8041.6261001.2891.3391.3141.136500.8240.8870.8560.678250.5580.5970.5780.40012.50.4920.4720.4820.3046.250.3080.2970.3030.12500.1840.1710.178 | |||||||||||||
货期：3-5 working days
×
