首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠抑制素A(INH-A)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的INH-A含量。试剂盒检测范围为0.5 pg/mL-100 pg/mL，适用于小鼠生殖生理机制研究、卵巢功能评估、生殖相关疾病模型构建等科研领域，为生殖生物学、发育生物学及内分泌学等基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Inha ELISA Kit; Inhibin alpha chain ELISA Kit
缩写：INH-A
Uniprot No.：Q04997
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.5 pg/mL-100 pg/mL
灵敏度：0.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse INH-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 101-109 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 83-90 | |||||
回收率：
| The recovery of mouse INH-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-87 | ||||
| EDTA plasma (n=4) | 101 | 98-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 1000.1470.1490.148 250.3010.3160.309 80.4590.4680.464 20.8510.8270.839 0.51.3951.4051.400 02.4682.4592.464 | ||||||
货期：3-5 working days
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