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Pig inducible nitric oxide synthase(iNOS)ELISA Kit

  • 中文名： 猪诱导型一氧化氮合成酶(iNOS)ELISA Kit
货号: IPD-E17489p
Price： ￥3000
数量：
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产品详情

  • 产品描述：猪诱导型一氧化氮合成酶(iNOS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的iNOS含量。iNOS即诱导型一氧化氮合酶，在炎症、免疫反应等生理病理过程中发挥重要作用。其背景在于它可被多种细胞因子和脂多糖等诱导表达。研究机制主要聚焦于其调控NO生成，过量NO与组织损伤、肿瘤等疾病相关，是潜在治疗靶点。试剂盒检测范围为0.05 U/mL-40 U/mL，适用于动物疾病模型构建、免疫调节机制研究、感染性疾病病理分析等领域，为探究炎症相关疾病的分子机制、评估实验性治疗方案的调控效果提供可靠工具，满足科研人员对iNOS动态变化的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Nos2 ELISA Kit; InoslNitric oxide synthase ELISA Kit; inducible ELISA Kit; EC 1.14.13.39 ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; Macrophage NOS ELISA Kit; MAC-NOS ELISA Kit; NOS type II ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit

  • 缩写：iNOS

  • Uniprot No.：P29477

  • 种属：Sus scrofa (Pig)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：0.05 U/mL-40 U/mL

  • 灵敏度：0.05 U/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Metabolism

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of pig iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)

    1:1Average %87

    Range %81-95

    1:8Average %93

    Range %89-99

    1:4Average %87

    Range %83-90

    1:8Average %89

    Range %84-100

  • 回收率：

    The recovery of pig iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)9286-100

    EDTA plasma (n=4)9381-99

















  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    U/mlOD1OD2AverageCorrected  401.8181.8061.8121.666  13.31.6521.6351.6441.498  4.441.4471.4291.4381.292  1.480.9870.9630.9750.829  0.490.6650.6480.6570.511  0.160.3680.3550.3620.216  0.050.2490.2440.2470.101  00.1450.1460.146　  







  • 货期：3-5 working days

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