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产品描述：鸡胰岛素（Insulin）酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的INS含量。INS即胰岛素基因，与血糖调节密切相关。其背景在于胰岛素是调节血糖的关键激素，INS功能异常会引发糖尿病等疾病。研究机制主要围绕基因表达调控、胰岛素合成与分泌过程等，旨在阐明发病机理，为糖尿病等相关疾病的治疗提供新靶点和策略。试剂盒检测范围为3.12 μIU/mL-50 μIU/mL，适用于科研领域家禽代谢调控机制研究，例如糖尿病模型构建、营养代谢相关疾病探索或药物干预后胰岛素分泌动态监测等场景，为禽类生理学、病理学及药理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
缩写：INS
Uniprot No.：P67970
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 μIU/mL-50 μIU/mL
灵敏度：0.59 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken insulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 95-103 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of chicken insulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
| EDTA plasma (n=4) | 97 | 95-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2Average 500.1410.1450.143 250.3100.3130.312 12.50.6850.6760.681 6.251.4771.4721.475 3.122.7092.7072.708 | ||||||
货期：3-5 working days
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