首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰岛素(INS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的INS含量。INS即胰岛素基因，其编码的胰岛素是调节血糖的关键激素。在糖代谢中，胰岛素与靶细胞受体结合，激活下游信号通路，促进细胞摄取葡萄糖，降低血糖。目前针对INS靶点的研究集中在糖尿病治疗，旨在通过调控基因表达或模拟其功能改善血糖控制。试剂盒检测范围为15.6 nIU/mL-1000 nIU/mL，适用于科研领域探究胰岛素分泌机制、评估代谢疾病动物模型、监测体外细胞实验的胰岛素释放动态等场景，特别适用于糖尿病机制研究、药物干预效果评价及细胞水平的功能性实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ins1 ELISA Kit; Ins-1 ELISA Kit; Insulin-1 [Cleaved into: Insulin-1 B chain; Insulin-1 A chain] ELISA Kit
缩写：INS
Uniprot No.：P01322
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates
检测范围：15.6 nIU/mL-1000 nIU/mL
灵敏度：3.9 nIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:400 | Average % | 84 | ||||
| Range % | 80-89 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of rat INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nIU/mlOD1OD2AverageCorrected 10002.3722.2342.3032.150 5001.8591.8121.8361.683 2501.4291.3651.3971.244 1251.0271.0971.0620.909 62.50.5760.5890.5830.430 31.20.3410.3170.3290.176 15.60.2130.2070.2100.057 00.1550.1510.153 | ||||||
货期：3-5 working days
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