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产品描述：猪胰岛素(INS)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的INS含量。INS为胰岛素基因，其编码的胰岛素是调节血糖的关键激素。在研究机制上，它参与糖代谢过程，维持血糖平衡。胰岛素分泌异常可能引发糖尿病等疾病，因此围绕INS的功能、调控及相关治疗靶点的研究，对代谢疾病防治意义重大。试剂盒检测范围为2 μIU/mL-400 μIU/mL，适用于糖尿病发病机制研究、胰岛素分泌功能评估、代谢类药物或饲料添加剂的药效学分析，以及动物实验中胰腺组织INS表达水平的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
缩写：INS
Uniprot No.：P01315
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：2 μIU/mL-400 μIU/mL
灵敏度：1 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 84-105 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-101 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 83-103 | |||||
回收率：
| The recovery of pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-101 | ||||
| EDTA plasma (n=4) | 98 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2Average 01.8001.8641.832 21.5691.5811.575 81.3041.3271.316 320.8120.8050.809 1000.3890.3930.391 4000.1800.1830.182 | ||||||
货期：3-5 working days
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