首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：马胰岛素(INS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的INS含量。INS即胰岛素基因，其编码的胰岛素是调节血糖的关键激素。在背景上，胰岛素分泌异常与糖尿病等疾病密切相关。研究机制方面，围绕其基因表达调控、蛋白合成加工及分泌等展开，旨在揭示疾病发病机制，为糖尿病等治疗提供理论基础。试剂盒检测范围为1.56 nIU/mL-100 nIU/mL，该产品可应用于动物科研领域，例如研究马的糖代谢调控机制、胰岛素相关疾病模型构建、营养干预或药物作用下的胰岛素分泌变化分析，以及运动生理学中能量代谢的动态监测等方向，为探索胰岛素在马体内的生物学功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
缩写：INS
Uniprot No.：P01310
种属：Equus caballus (Horse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 nIU/mL-100 nIU/mL
灵敏度：0.39 nIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of horse INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:2000 | Average % | 87 | ||||
| Range % | 84-91 | |||||
| 1:4000 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8000 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of horse INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-92 | ||||
| EDTA plasma (n=4) | 104 | 100-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nIU/mlOD1OD2AverageCorrected1002.6592.6982.6792.546502.3532.3012.3272.194251.9221.9041.9131.78012.51.1791.1981.1891.0566.250.7020.7320.7170.5843.120.4110.4350.4230.2901.560.3050.3300.3180.18500.1320.1340.133 | ||||||
货期：3-5 working days
×
