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产品描述：豚鼠胰岛素(INS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的INS含量。INS 即胰岛素基因，其编码的胰岛素是调节血糖的关键激素。在研究机制上，主要聚焦于其表达调控、胰岛素信号通路等。深入研究有助于理解糖尿病等代谢疾病发病机制，也为开发相关治疗药物，如胰岛素类似物等提供理论基础。试剂盒检测范围为12 μIU/mL-210 μIU/mL，适用于豚鼠模型中胰岛素相关科研分析；在糖尿病机制研究、代谢性疾病模型构建及药物干预效果评价等领域具有重要应用价值；可为胰腺功能研究、胰岛素分泌调控实验提供精准数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
缩写：INS
Uniprot No.：P01329
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：12 μIU/mL-210 μIU/mL
灵敏度：7.5 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-106 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 86-104 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 87-94 | |||||
回收率：
| The recovery of guinea pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-101 | ||||
| EDTA plasma (n=4) | 95 | 89-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2Average 120.0490.0510.050 240.1650.1600.163 480.3770.3740.376 1200.8530.8490.851 2101.6861.6911.689 | ||||||
货期：3-5 working days
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