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产品描述：鱼胰岛素(INS)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的INS含量。INS即胰岛素基因，其表达产生的胰岛素在调节血糖稳态中至关重要。研究机制方面，围绕胰岛素的分泌、作用信号通路等展开，异常的INS表达或功能与糖尿病等代谢疾病密切相关，对其深入研究有助于揭示疾病发病机制及开发新疗法。试剂盒检测范围为100 pg/mL-1600 pg/mL，该产品适用于鱼类生理学研究，例如评估不同生长阶段、营养状态或环境胁迫（如水温、盐度变化）对胰岛素分泌的影响，也可用于水产养殖中鱼类代谢疾病模型的机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：INS
Uniprot No.：P04667?
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：100 pg/mL-1600 pg/mL
灵敏度：100 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-100 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of fish INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-98 | ||||
| EDTA plasma (n=4) | 92 | 90-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 16000.1780.1890.184 8000.3220.3400.331 4000.6100.6340.622 2001.2571.2301.244 1002.3352.4752.405 | ||||||
货期：3-5 working days
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