首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胰岛素(INS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的INS含量。INS即胰岛素基因，在人体糖代谢中极为关键。它编码的胰岛素可促进细胞摄取葡萄糖，降低血糖水平。研究主要围绕其表达调控机制展开，以了解胰岛素分泌异常与糖尿病等疾病关联，为开发治疗药物和方案提供理论依据。试剂盒检测范围为15.6 nIU/mL-1000 nIU/mL，该产品适用于科研场景中评估胰岛β细胞功能、分析胰岛素分泌调控机制或筛选影响糖代谢的化合物，例如在建立糖尿病动物模型后监测胰岛素动态变化，或通过体外细胞实验探究药物对胰岛素合成与释放的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ins1 ELISA Kit; Ins-1 ELISA Kit; Insulin-1 [Cleaved into: Insulin-1 B chain; Insulin-1 A chain] ELISA Kit
缩写：INS
Uniprot No.：P01325
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：15.6 nIU/mL-1000 nIU/mL
灵敏度：3.9 nIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 84-95 | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of mouse INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-102 | ||||
| EDTA plasma (n=4) | 89 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nIU/ml.OD1OD2AverageCorrected 10002.5462.5982.5722.396 5002.2312.2672.2492.073 2501.7451.7691.7571.581 1251.1981.1631.1811.005 62.50.6480.6280.6380.462 31.20.4260.4070.4170.241 15.60.3020.3260.3140.138 00.1730.1790.176 | ||||||
货期：3-5 working days
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