首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胰岛素样肽INSL5(INSL5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的INSL5含量。INSL5，即胰岛素样肽5，是胰岛素超家族的一员，具有胰岛素家族的经典结构。其研究机制主要涉及与受体RXFP4的相互作用，调节能量代谢、食欲调节和肠道蠕动等生理功能。近期研究发现，INSL5在肿瘤糖代谢调控中发挥重要作用，通过激活STAT5信号通路促进糖酵解相关基因的表达，影响细胞糖代谢重编程，进而促进肿瘤进展。试剂盒检测范围为3.12 pg/mL-200 pg/mL，可为研究INSL5在代谢综合征、食欲异常调控、胃肠道功能紊乱等领域的分子机制提供可靠工具，也可用于评估药物干预或基因修饰模型中INSL5的表达变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Insl5 ELISA Kit; Rif ELISA Kit; Rif2 ELISA Kit; Zins3 ELISA Kit; Insulin-like peptide INSL5 ELISA Kit; Insulin-like peptide 5 ELISA Kit; Relaxin/insulin-like factor 2 ELISA Kit; Relaxin/insulin-like protein) [Cleaved into: Insulin-like peptide INSL5 B chain; Insulin-like peptide INSL5 A chain] ELISA Kit
缩写：INSL5
Uniprot No.：Q9WUG6
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse INSL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:200 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:400 | Average % | 88 | ||||
| Range % | 85-93 | |||||
| 1:800 | Average % | 107 | ||||
| Range % | 103-110 | |||||
回收率：
| The recovery of mouse INSL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 81-89 | ||||
| EDTA plasma (n=4) | 98 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.4172.4462.4322.2621002.0462.0082.0271.857501.5621.5681.5651.395251.0171.0351.0260.85612.50.6920.6690.6810.5116.250.4250.4350.4300.2603.120.3060.3300.3180.14800.1690.1710.170 | ||||||
货期：3-5 working days
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